vitamin d gene expression
J Korean Med Sci. In vitro, vitamin D increases expression of the tyrosine hydroxylase gene in adrenal medullary cells, and affects the synthesis of neurotrophic factors, nitric oxide synthase, and glutathione. 2004;10(21):2535-55. doi: 10.2174/1381612043383818. Zhuang SH, Schwartz GG, Cameron D, Burnstein KL. Vitamin D insufficiency was defined as concentrations lower than 75 nmol/L (nanomoles per liter) or 50 nmol/L. Vitamin D plays a key role in the expression of our genes, and therefore, in the many complex cellular pathways involved throughout the body, in all systems of health. Recently, however, a series of technical advances involving the coupling of chromatin immunoprecipitation (ChIP) to unbiased methodologies that initially involved tiled DNA microarrays (ChIP-chip analysis) and now Next Generation DNA Sequencing techniques (ChIP-seq analysis) has opened new avenues of research into the mechanisms through which 1,25(OH)2D3 regulates gene expression. Bethesda, MD 20894, Copyright Iran Biomed J. In this review, we summarize briefly the results of this early work and then focus on more recent studies in which ChIP-chip and ChIP-seq analyses have been used to explore the mechanisms of 1,25(OH)2D3 action on a genome-wide scale providing specific target genes as examples. Specifically, addition or removal of methyl groups to phosphate-linked cytosine–guanine (CpG) dinucleotides (i.e., DNA methylation) has been implicated as a potential mechanism linking in utero and early life vitamin D status to long-term offspring gene expression (reviewed by Wright et al. National Library of Medicine The Journal of Steroid Biochemistry and Molecular Biology, https://doi.org/10.1016/j.jsbmb.2013.11.004. Design Expression of vitamin D receptor (VDR) and two 1,25(OH) 2 D 3 target genes was analysed in 658 patients with CRC with prolonged clinical follow-up. As most diets are severely lacking in vitamin D, the most practical way of getting adequate vitamin D is by taking an inexpensive daily, oral D3 supplement. Copyright © 2021 Elsevier B.V. or its licensors or contributors. The vitamin D receptor is a protein activated by vitamin D, which attaches itself to DNA and thus influences what proteins are made from our genetic code… In vitro, vitamin D treatment reduced GM-CSF expression (17), and increased the expression of FOXP3 and IL-10 (18, 19), suggesting that vitamin D is involved in restraining T cell responses. Furthermore, we studied for the first time whether VDR gene expression in AT was related to diabetes, finding a trend toward a higher VDR gene expression in P&D compared to NG subjects. The molecular details involved in vitamin D-regulated gene expression by the VDR in key mineral-regulating tissues such as the intestine, kidney, and bone are revealing important insights into the manner in which our bodies maintain the structural integrity of skeletal tissue. The combination of vitamin D3 and dehydroascorbic acid administration attenuates brain damage in focal ischemia. 8600 Rockville Pike PCR array analysis of 92 muscle genes identified vitamin D receptor (VDR) mRNA in all muscle biopsies, with this expression being negatively correlated with serum 25OHD3, and Vmax, and positively correlated with fat mass. Initial research focused upon several known genetic targets provided early insight into the mechanism of action of the vitamin D hormone (1,25-dihydroxyvitamin D3 (1,25(OH)2D3)). The active form of vitamin D functions as a steroid hormone. Osteoblast differentiation and control by vitamin D and vitamin D metabolites. The molecular basis of vitamin D signaling implies that the metabolite 1α,25-dihydroxyvitamin D 3 (1,25 (OH) 2 D 3) of the secosteroid vitamin D 3 activates the transcription factor vitamin D receptor (VDR), which in turn modulates the expression of hundreds of primary vitamin D target genes. This study validates the importance of vitamin D to human health beyond its role in calcium metabolism and bone health. This means that given the same levels of vitamin D, someone with the short ‘C’ allele VDR protein will display increased expression of vitamin D dependent genes (1, 2). 2009 Jun;30(3):207-12. doi: 10.1007/s10072-009-0038-6. J Steroid Biochem Mol Biol. Association of vitamin D receptor gene polymorphism and Parkinson's disease in Koreans. Prevention and treatment information (HHS). To investigate how the gene axis is affected by maternal vitamin D status, Lee's team studied placental samples from 43 pregnant women enrolled … Mol Cell Endocrinol. Would you like email updates of new search results? 1,25(OH) 2 D 3 effects on primary cultures of patient-derived colon normal fibroblasts (NFs) and cancer-associated fibroblasts (CAFs) were studied using collagen gel contraction and migration assays and global gene expression analyses. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. 1996;19(6):325-31. doi: 10.1159/000174095. Epub 2014 Mar 1. Curr Pharm Des. Neurol Sci. This site needs JavaScript to work properly. 2014 Oct;144 Pt A:189-96. doi: 10.1016/j.jsbmb.2013.11.013. Careers. Vitamin D deficiency has been associated with increased incidence of diabetes, both in humans and in animal models. Vitamin D is necessary for building and maintaining healthy bones. The actions of the vitamin D hormone 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) are mediated by the vitamin D receptor (VDR), a ligand-activated transcription factor that functions to control gene expression. Participants were randomly assigned into two intervention groups for receiving either 50,000 IU vitamin D or placebo (n = 20 each group) every other week for 8 weeks. The DNA sequence to which the VDR binds is comprised of a classic VDRE. In addition, an association between vitamin D receptor (VDR) gene polymorphisms and diabetes has also been described. CYP24A1 is expressed in tissues which are considered targets for vitamin D, including kidney, intestine and bone. VDR binding regions are modular and marked by specific histone modifications. COVID-19 is an emerging, rapidly evolving situation. The research highlights a direct link between circulating vitamin D levels and the expression of a gene called ID1, known to be associated with tumor growth and breast cancer metastasis. Maund SL, Barclay WW, Hover LD, Axanova LS, Sui G, Hipp JD, Fleet JC, Thorburn A, Cramer SD. J Neurooncol. Clipboard, Search History, and several other advanced features are temporarily unavailable. VDR binding sites are located predominantly within introns and intergenic regions. 2014 May;118(1):49-60. doi: 10.1007/s11060-014-1416-3. Fundamentals of vitamin D hormone-regulated gene expression, silencing mediator retinoid and thyroid hormones, transcription activator-like effector nucleases, clustered regularly interspersed short palindromic repeats. Accessibility Unable to load your collection due to an error, Unable to load your delegates due to an error. The T(f) allele of the functional SNP FokI, in exon 2 of VDR, results in a longer vitamin D receptor protein (VDR) isoform, proposed to be less active. By continuing you agree to the use of cookies. VDR DNA binding is dependent upon 1,25(OH)2D3 and generally involves RXR. Epub 2013 Nov 26. 2011 Aug 1;71(15):5276-86. doi: 10.1158/0008-5472.CAN-10-2160. 2013;17(1):42-8. doi: 10.6091/ibj.1061.2012. We use cookies to help provide and enhance our service and tailor content and ads. 1,25-dihydroxyvitamin D3 and the synthetic vitamin D analogue, KH 1060, modulate the growth of mouse proximal tubular cells. To investigate how the gene axis is affected by maternal vitamin D status, Lee's team studied placental samples from 43 pregnant women enrolled … So, yeah, it’s pretty important. Vitamin D3 supplementation that improved serum 25-hydroxyvitamin D concentrations was associated with at least a 1.5 fold alteration in the expression of 291 genes. Researchers found that vitamin D sufficiency lowered the expression of placental genes related to preeclampsia - a severe, and sometimes fatal, condition. Transcription of the CYP24A1 gene is markedly inducible by 1,25- (OH) 2 D 3 binding to the vitamin D receptor. This article is part of a Special Issue entitled ‘16th Vitamin D Workshop’. Keratinocytes of the epidermis possess the enzymes needed to convert vitamin D to its active form, 1,25-dihydroxyvitamin D 3 , as well as the vitamin D receptor (VDR), a transcription factor that regulates gene expression. Please enable it to take advantage of the complete set of features! The vitamin D hormone 1,25 (OH) 2 D 3 exerts its diverse biological effects in target tissues by regulating gene expression. Epub 2009 Mar 6. Vitamin D vital for gene expression in developing brains by Samantha Saw, Science Network WA The study investigated the gene expression of four neurotrophic genes responsible … Kim JS, Kim YI, Song C, Yoon I, Park JW, Choi YB, Kim HT, Lee KS. Copyright © 2013 Published by Elsevier Ltd. Vitamin D receptor content and transcriptional activity do not fully predict antiproliferative effects of vitamin D in human prostate cancer cell lines. Epub 2011 Jun 8. Interleukin-1α mediates the antiproliferative effects of 1,25-dihydroxyvitamin D3 in prostate progenitor/stem cells. Weinreich T, Müller A, Wüthrich RP, Booy C, Binswanger U. Kidney Blood Press Res. Salomón DG, Fermento ME, Gandini NA, Ferronato MJ, Arévalo J, Blasco J, Andrés NC, Zenklusen JC, Curino AC, Facchinetti MM. Gene expression for insulin and lipid metabolism was conducted using peripheral blood mononuclear cells (PBMCs) of … However, the involvement of VDR in the development of diabetes, specifically in pancreatic β-cells, has not been elucidated yet. The Gene Expression Omnibus public repository was searched for studies using the keyword “vitamin D.” All gene expression studies examining the effects of vitamin D and its metabolites or calcitriol analogs, for which the raw data were available, were included. Most of these actions are mediated by activation of the nuclear vitamin D receptor, which regulate … The gene has a strong, positive vitamin D response element in the promoter. Vitamin D receptor expression is associated with improved overall survival in human glioblastoma multiforme. Considerable progress has been made in the understanding of vitamin D receptor function (especially regarding its interaction with coactivators), as well as in the identification of novel vitamin D responsive genes related to cell growth, differentiation and cytokine production. The function of 1,25-dihydroxyvitamin D3, the biologically active form of vitamin D, extends from bone and mineral homeostasis to the control of cell growth and differentiation in a variety of tissues. 2005 Jun;20(3):495-8. doi: 10.3346/jkms.2005.20.3.495. Studies in VDR knockout mice were excluded from the analysis. 15). The results of this work have advanced our understanding of the mechanisms involved at both genetic and epigenetic levels and have revealed a series of new principles through which the vitamin D hormone functions to control the expression of genes. Vitamin D and regulation of gene expression The function of 1,25-dihydroxyvitamin D3, the biologically active form of vitamin D, extends from bone and mineral homeostasis to the control of cell growth and differentiation in a variety of tissues. In vitro studies have shown that 1,25(OH) 2 D 3 influences VDR gene expression and adipogenesis . 1997 Jan 3;126(1):83-90. doi: 10.1016/s0303-7207(96)03974-3. Vitamin D deficiency impairs spatial learning in adult rats. Eldecalcitol replaces endogenous calcitriol but does not fully compensate for its action in vivo. Novel genome-wide approaches provide new insight into the actions of 1,25(OH)2D3. Hinari - Access to Research for Health programme. Common Vitamin D Genetic Variants. Many epidemiological studies have shown that vitamin D deficiency is associated with high CRC risk and mortality, which suggests that vitamin D has a protective effect against this disease 20, 21. The most active vitamin D metabolite 1α,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 , calcitriol) is a major regulator of gene expression in humans. Cancer Res. Polymorphisms of the vitamin D receptor gene (VDR) have been associated inconsistently with various diseases, across populations of diverse origin. In fact, vitamin D controls the expression of about 3% of the human genome. Additionally, vitamin D can act as a hormone within cells to immediately cause non-DNA related actions to take place – such as opening ion channels or causing the secretion of insulin. The VDR gene provides instructions for making a protein called vitamin D receptor (VDR), which allows the body to respond appropriately to vitamin D. This vitamin can be acquired from foods in the diet or made in the body with help from sunlight. Most of these actions are mediated by activation of the nuclear vitamin D receptor, which regulates the transcription of vitamin D target genes. Two common vitamin D-related gene variants are VDR taq and VDR bsm. That's because calcium, the primary component of bone, can only be absorbed by your body when vitamin D is present. There was a significant difference in the expression of 66 genes between subjects at baseline with vitamin D deficiency (25 (OH)D<20 ng/ml) and subjects with a 25 (OH)D>20 ng/ml. Control of Gene Expression by Vitamin D Receptor: Full description or abstract: The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. Vitamin D gene expression associated with diseases – March 2013 No apparent genetic association between vitamin D and obesity – Feb 2013 Vitamin D level can be high, but little benefit: due to kidney, genes, low Magnesium etc. Privacy, Help FOIA It belongs to a family of ligand induced transcription factors, nuclear receptors (NRs).